p123shy1-mCherry-HA
(Plasmid
#67875)
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Purposeencodes shy1 with a C-terminal mCherry-HA tag under control of its endogenous promoter.It's suitable for localization studies in Ustilago maydis.
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Depositing Lab
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Depositing OrganizationMax Planck Institute for Terrestrial Microbiology
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 67875-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 67875-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep123
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Vector typeexpression in Ustilago maydis
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Selectable markersCarboxin resistance for selection in Ustilago maydis.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshy1
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Alt nameum05230
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SpeciesU. maydis
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Insert Size (bp)1330
- Promoter shy1
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Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pBRforEco
- 3′ sequencing primer mCherry-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
By linearizing it in the CBX gene it can be used for integration into the ip-locus of U.maydis.
DNA (Catalog # 67875-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 67875-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p123shy1-mCherry-HA was a gift from Armin Djamei (Addgene plasmid # 67875 ; http://n2t.net/addgene:67875 ; RRID:Addgene_67875) -
For your References section:
Degradation of the plant defence hormone salicylic acid by the biotrophic fungus Ustilago maydis. Rabe F, Ajami-Rashidi Z, Doehlemann G, Kahmann R, Djamei A. Mol Microbiol. 2013 Jul;89(1):179-88. doi: 10.1111/mmi.12269. Epub 2013 Jun 11. 10.1111/mmi.12269 PubMed 23692401
