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pSDMA57
(Plasmid #67941)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67941 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II -SK
  • Backbone manufacturer
    Stratagene
  • Modifications to backbone
    Added Safe Haven gene free region and NEO resistance marker and removed F1 origin
  • Vector type
    Cryptococcal complementation vector
  • Promoter ACT1 for Cryptococcal marker
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains the safe haven site sequence in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the safe haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes—BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSDMA57 was a gift from James Fraser (Addgene plasmid # 67941 ; http://n2t.net/addgene:67941 ; RRID:Addgene_67941)
  • For your References section:

    A genomic safe haven for mutant complementation in Cryptococcus neoformans. Arras SD, Chitty JL, Blake KL, Schulz BL, Fraser JA. PLoS One. 2015 Apr 9;10(4):e0122916. doi: 10.1371/journal.pone.0122916. eCollection 2015. PONE-D-15-02673 [pii] PubMed 25856300