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pABD1031
(Plasmid #68004)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68004 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSITE-BiFC-C1cec
  • Backbone manufacturer
    Martin et al 2009 The Plant Journal, 59 150-162 http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2009.03850.x
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 9439
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 20 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PHY-INTERACTING FACTOR 1 (PIF1)
  • Alt name
    PHYTOCHROME INTERACTING FACTOR 3-LIKE 5, PIL5
  • Insert Size (bp)
    1439
  • GenBank ID
    AT2G20180
  • Entrez Gene
    PIL5 (a.k.a. AT2G20180, PHY-INTERACTING FACTOR 1, PIF1, T2G17.2, T2G17_2, phytochrome interacting factor 3-like 5)
  • Promoter 2X Cauliflower Mosaic Virus 35S promoter
  • Tag / Fusion Protein
    • eYFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pABD1031 was a gift from Bruce Downie (Addgene plasmid # 68004 ; http://n2t.net/addgene:68004 ; RRID:Addgene_68004)
  • For your References section:

    KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1. Majee M, Kumar S, Kathare PK, Wu S, Gingerich D, Nayak NR, Salaita L, Dinkins R, Martin K, Goodin M, Dirk LMA, Lloyd TD, Zhu L, Chappell J, Hunt AG, Vierstra R, Huq E, Downie AB. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 10.1073/pnas.1711919115. Epub 2018 Apr 9. 10.1073/pnas.1711919115 PubMed 29632208