pcDNA3.1dsRluc
(Plasmid
#68053)
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Purposeexpresses destabilized Renilla Luciferase
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepcDNA3.1+
- Backbone size w/o insert (bp) 5428
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Modifications to backboneneomycin resistance replaced with puromycin resistance.
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla luciferase
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1dsRluc was a gift from Christopher Sullivan (Addgene plasmid # 68053 ; http://n2t.net/addgene:68053 ; RRID:Addgene_68053) -
For your References section:
Evolutionarily conserved function of a viral microRNA. Seo GJ, Fink LH, O'Hara B, Atwood WJ, Sullivan CS. J Virol. 2008 Oct;82(20):9823-8. doi: 10.1128/JVI.01144-08. Epub 2008 Aug 6. 10.1128/JVI.01144-08 PubMed 18684810