pBLV B4
(Plasmid #68057)

• Purpose: expresses BLV (NC001414) B4 pre-miRNA and miRNAs
• Depositing Lab: Christopher Sullivan
• Publication: Burke et al Nucleic Acids Res. 2014 Dec 16;42(22):13949-62. doi: 10.1093/nar/gku1247. Epub 2014 Nov 26.

Backbone

• Vector backbone: pIDTSmart-Kan
• Backbone manufacturer: Integrated DNA Technologies
• Backbone size w/o insert (bp): 1962
• Total vector size (bp): 2013
• Modifications to backbone: Xho1 and Xba1 sites were inserted into the pIDTSmart-Kan backbone.
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BLV-miR-B4
• gRNA/shRNA sequence: N/A
• Species: Bovine leukemia virus
• GenBank ID: NC_001414

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: M13 Foward
• 3′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry