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(Plasmid #68121)


Item Catalog # Description Quantity Price (USD)
Plasmid 68121 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Currently unavailable outside the U.S.

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Bernhard Moss, National Institutes of Health
  • Backbone size w/o insert (bp) 5357
  • Total vector size (bp) 6743
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    VP35 ZEBOV
  • Insert Size (bp)
  • Entrez Gene
    VP35 (a.k.a. ZEBOVgp2)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer ATT GTA TGG GAT CTG ATC TGG
  • 3′ sequencing primer GCC AAC TCA GCT TCC TTT CGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTM1_VP35_ZEBOV was a gift from Elke Mühlberger (Addgene plasmid # 68121 ; ; RRID:Addgene_68121)
  • For your References section:

    The L-VP35 and L-L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals. Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, Muhlberger E. Virology. 2013 Jul 5;441(2):135-45. doi: 10.1016/j.virol.2013.03.013. Epub 2013 Apr 11. 10.1016/j.virol.2013.03.013 PubMed 23582637