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(Plasmid #69359)


Item Catalog # Description Quantity Price (USD)
Plasmid 69359 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Currently unavailable outside the U.S.

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Andrew Ball, University of Alabama at Birmingham
  • Backbone size w/o insert (bp) 3077
  • Total vector size (bp) 5016
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    3E5E eGFP minigenome
  • Insert Size (bp)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site RsRII (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer ATT GTA TGG GAT CTG ATC TGG
  • 3′ sequencing primer GCC AAC TCA GCT TCC TTT CGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2,0_3E5E_eGFP was a gift from Elke Mühlberger (Addgene plasmid # 69359 ; ; RRID:Addgene_69359)
  • For your References section:

    Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread. Schmidt KM, Schumann M, Olejnik J, Krahling V, Muhlberger E. J Infect Dis. 2011 Nov;204 Suppl 3:S861-70. doi: 10.1093/infdis/jir308. 10.1093/infdis/jir308 PubMed 21987762