Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCer-C3-Plk1-T210D
(Plasmid #68133)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68133 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6500
  • Modifications to backbone
    EGFP swapped for Cerulean
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Polo-like kinase 1
  • Alt name
    Plk1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Mutation
    T210D constitutively active version
  • GenBank ID
    NM_005030.4
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Promoter CMV
  • Tag / Fusion Protein
    • Cerulean (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (destroyed during cloning)
  • 5′ sequencing primer GFP-FP
  • 3′ sequencing primer pEGFP-C2-RP
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCer-C3-Plk1-T210D was a gift from Catherine Lindon (Addgene plasmid # 68133 ; http://n2t.net/addgene:68133 ; RRID:Addgene_68133)