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pTXB3-Flag PLK1 T210D
(Plasmid #68272)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68272 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTXB1
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PLK1
  • Alt name
    polo-like kinase 1
  • Species
    H. sapiens (human)
  • Mutation
    T210D
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Promoter T7
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • Mxe intein/chitin binding domain (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Use BL21 cells for protein production

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXB3-Flag PLK1 T210D was a gift from Stefano Ferrari (Addgene plasmid # 68272 ; http://n2t.net/addgene:68272 ; RRID:Addgene_68272)