PurposeCRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68414||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
Vector typeLentiviral, Luciferase
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCypridina Luciferase-2A-Venus YFP
MutationYFP: M153T,V163A,S175G ("Venus") YFP Variant
- Promoter minimalCMV
- Cloning method Gibson Cloning
- 5′ sequencing primer LNCX
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Reporters are separated by 2A "self-cleaving" peptides. The reporter cassette is under the control of a minimal CMV promoter, preceeded by a casette of nine (ATCTAGATCGCCCGTCCCCT)AGG sites. The Tet-reponsive promoter and Gateway cloning sites were removed from the parent vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti_9xD3B_Cluc_Norm was a gift from John Rinn (Addgene plasmid # 68414 ; http://n2t.net/addgene:68414 ; RRID:Addgene_68414)
For your References section:Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. 10.1038/nmeth.3433 PubMed 26030444