PurposeTransient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68418||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
MutationPP7: [delta]67_74 (non-aggregating)
- Promoter human UbC
/ Fusion Proteins
- HA (N terminal on insert)
- VP64 (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer hUBCpro-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Contains an N-terminal 3xNLS cassette. Contains two tandem copies of the PP7 protein, connected by a flexible linker
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUBC_HA_2xPP7-VP64 was a gift from John Rinn (Addgene plasmid # 68418 ; http://n2t.net/addgene:68418 ; RRID:Addgene_68418)
For your References section:Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. 10.1038/nmeth.3433 PubMed 26030444