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EGFPC2-CB2 SH3+
(Plasmid #68821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68821 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 68821-D50 50 μg of DNA in Tris buffer $495
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DNA 68821-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Collybistin
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1236
  • Mutation
    none
  • Entrez Gene
    Arhgef9
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CB2SH3+ contains the SH3 domain splice cassette, CB2SH3- lacks the Sh3 domain splice cassette. That is the only difference between these two splice isoforms.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFPC2-CB2 SH3+ was a gift from Shiva Tyagarajan (Addgene plasmid # 68821 ; http://n2t.net/addgene:68821 ; RRID:Addgene_68821)

  • For your References section:

    RhoGEF9 splice isoforms influence neuronal maturation and synapse formation downstream of alpha2 GABAA receptors. de Groot C, Floriou-Servou A, Tsai YC, Fruh S, Kohler M, Parkin G, Schwerdel C, Bosshard G, Kaila K, Fritschy JM, Tyagarajan SK. PLoS Genet. 2017 Oct 25;13(10):e1007073. doi: 10.1371/journal.pgen.1007073. eCollection 2017 Oct. 10.1371/journal.pgen.1007073 PubMed 29069083
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