pCR3-FLAG-Gephyrin P1
(Plasmid #68816)

• Purpose: Flag tagged Geph mammalian expression
• Depositing Lab: Shiva Tyagarajan
• Publication: Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47. doi: 10.1074/jbc.M112.442616. Epub 2013 Feb 13.

Backbone

• Vector backbone: pCR3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 7300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gephyrin
• Alt name: GPHN
• Species: R. norvegicus (rat)
• Insert Size (bp): 2200
• Mutation: silent mutations on internal EcoRI site. Gephyrin D566N (see depositor comment below)
• GenBank ID: NM022865.3
• Entrez Gene: Gphn (a.k.a. Geph)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gephyrin D566N mutation is a polymorphism and is not a concern for plasmid function.