Purposedeleted OPTN was inserted into pEGFP-N1 backbone
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68838||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4733
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationaa 1-55 only
Entrez GeneOPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Wild type construct is pOPTN-EGFP (Plasmid #27052)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOPTN1-55-EGFP was a gift from Beatrice Yue (Addgene plasmid # 68838 ; http://n2t.net/addgene:68838 ; RRID:Addgene_68838)
For your References section:Effects of mutations and deletions in the human optineurin gene. Turturro S, Shen X, Shyam R, Yue BY, Ying H. Springerplus. 2014 Feb 19;3:99. doi: 10.1186/2193-1801-3-99. eCollection 2014. 845 [pii] PubMed 24683533