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GST-TPX2(1-43)
(Plasmid #69107)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69107 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-6p-1
  • Backbone size w/o insert (bp) 4981
  • Total vector size (bp) 5128
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Targeting Protein for Xklp2
  • Alt name
    TPX2, microtubule-associated
  • Species
    H. sapiens (human)
  • Mutation
    Amino acids 1-43 expressed.
  • Entrez Gene
    TPX2 (a.k.a. C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert amplified from IMAGE clone (ID unknown).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-TPX2(1-43) was a gift from Stephen Royle (Addgene plasmid # 69107 ; http://n2t.net/addgene:69107 ; RRID:Addgene_69107)
  • For your References section:

    The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Nixon FM, Gutierrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle SJ. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. 10.7554/eLife.07635 PubMed 26090906