mCherry-Incenp wt
(Plasmid #108487)

• Purpose: expression of vsv-INCENP wt-mCherry
• Depositing Lab: Susanne Lens
• Publication: Liu et al Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15.

Backbone

• Vector backbone: mCherry-N1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INCENP
• Species: H. sapiens (human)
• Insert Size (bp): 2757
• Entrez Gene: INCENP
• Promoter: CMV
• Tags / Fusion Proteins:
  • VSV (N terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HinDIII (unknown if destroyed)
• 3′ cloning site: HinDIII (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • SL-439 vsv-Incenp WT-mCherry -ATG.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry