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Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates.
Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM
Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
45227Cenp-B INCENP Δcen mCherry
45228Mis12 INCENP Δcen mCherry
45229nonphosphorylatable Mis12-targeted Aurora B FRET sensor
45230nonphosphorylatable Cenp-B-targted Aurora B FRET sensor
45231Mis12-targted Aurora B FRET sensor
45232Cenp-B-targeted Aurora B FRET sensor
108474pCR3-vsv-CenpB 1-166expression of vsv-CenpB 1-166
108475GFP-CenpB 1-166expression of GFP-CenpB 1-166
108477pCR3-vsv-Mis12-dCEN-Incenpexpression of vsv-Mis12-dCEN-INCENP
108478pEGFP-vsv-Mis12-dCEN-Incenp-EGFPexpression of vsv-Mis12-dCEN-INCENP-EGFP
108480pcDNA4-Tet-on-vsv-Incenpinducible expression of vsv-INCENP wt
108481pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenpinducible expression of vsv-CenpB-1-166-dCEN-INCENP
108482pcDNA4-Tet-on-vsv-Mis12-dCEN Incenpinducible expression of vsv-Mis12-dCEN-INCENP
108483pcDNA4-Tet-on-vsv-Incenp-EGFPinducible expression of vsv-INCENP wt-EGFP
108484pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFPinducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP
108485pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFPinducible expression of vsv-Mis12-dCEN-INCENP-EGFP
108486pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFPinducible expression of vsv-Mis12-dCEN-INCENP-EGFP
108487mCherry-Incenp wtexpression of vsv-INCENP wt-mCherry

Antibodies from Article