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His6-PRC1
(Plasmid #69111)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69111 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSET-B
  • Backbone size w/o insert (bp) 2803
  • Total vector size (bp) 4666
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein Regulator of Cytokinesis 1
  • Alt name
    PRC1
  • Alt name
    ASE1
  • Alt name
    MAP65
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1863
  • Entrez Gene
    PRC1 (a.k.a. ASE1)
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human PRC1 purchased as IMAGE clone (reference unknown).
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-PRC1 was a gift from Stephen Royle (Addgene plasmid # 69111 ; http://n2t.net/addgene:69111 ; RRID:Addgene_69111)
  • For your References section:

    The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Nixon FM, Gutierrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle SJ. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. 10.7554/eLife.07635 PubMed 26090906