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pBrain-ch-TOGKDP-GFP-shch-TOG
(Plasmid #69113)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69113 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBrain
  • Backbone size w/o insert (bp) 5447
  • Total vector size (bp) 11543
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Colonic Hepatic Tumour Over-expressed Gene
  • Alt name
    ch-TOG
  • Alt name
    CKAP5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6096
  • Mutation
    Silent mutations to confer shRNA resistance.
  • GenBank ID
    NM_001008938
  • Entrez Gene
    CKAP5 (a.k.a. CHTOG, MSPS, TOG, TOGp, ch-TOG)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site AfeI (not destroyed)
  • 3′ cloning site DraIII (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    shch-TOG
  • Species
    H. sapiens (human)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ch-TOG cDNA purchased. IMAGE ID: 40072423 (Clone BC120869).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBrain-ch-TOGKDP-GFP-shch-TOG was a gift from Stephen Royle (Addgene plasmid # 69113 ; http://n2t.net/addgene:69113 ; RRID:Addgene_69113)
  • For your References section:

    TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. 10.1242/bio.201410843 PubMed 25596274