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pNeoXTR f0
(Plasmid #69157)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69157 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 7775
  • Modifications to backbone
    see pNeoXTR f0.gb file
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    DTA
  • Species
    diptheria
  • Insert Size (bp)
    984
  • Promoter PGK

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    NeoR
  • Insert Size (bp)
    804
  • Promoter PGK

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    GFP
  • Alt name
    green fluorescent protein
  • Insert Size (bp)
    720
  • Promoter none

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    AmpR
  • Insert Size (bp)
    861
  • Promoter Amp promoter

Cloning Information for Gene/Insert 4

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNeoXTR f0 was a gift from David Feldser (Addgene plasmid # 69157 ; http://n2t.net/addgene:69157 ; RRID:Addgene_69157)
  • For your References section:

    Recombinase-based conditional and reversible gene regulation via XTR alleles. Robles-Oteiza C, Taylor S, Yates T, Cicchini M, Lauderback B, Cashman CR, Burds AA, Winslow MM, Jacks T, Feldser DM. Nat Commun. 2015 Nov 5;6:8783. doi: 10.1038/ncomms9783. 10.1038/ncomms9783 PubMed 26537451