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Fgf4enhLV (FpG12)
(Plasmid #69444)


Item Catalog # Description Quantity Price (USD)
Plasmid 69444 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Lois and Baltimore (ADDGENE # 14882)
  • Backbone size w/o insert (bp) 9206
  • Total vector size (bp) 11906
  • Modifications to backbone
    inserted FGF4 enhancer and minimal FGF4 promoter upstream of GFP coding sequences. Inserted human Ubiquitin promoter upstream of Hygromycin resistance gene. cloning details described in Murtha et al Nat Meth 2014
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
    minimal FGF4 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)

Gene/Insert 2

  • Gene/Insert name
  • Insert Size (bp)

Gene/Insert 3

  • Gene/Insert name
    Ubiquitin promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)

Gene/Insert 4

  • Gene/Insert name
    Hygromycin resistance
  • Insert Size (bp)

Gene/Insert 5

  • Gene/Insert name
    FGF4 enhancer
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Fgf4 (a.k.a. Fgf-4, Fgf7a, Fgfk, HBGF-4, Hst1, Hstf-1, KS3, hst, hst-1, kFGF)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fgf4enhLV (FpG12) was a gift from Lisa Dailey (Addgene plasmid # 69444 ; ; RRID:Addgene_69444)
  • For your References section:

    FIREWACh: high-throughput functional detection of transcriptional regulatory modules in mammalian cells. Murtha M, Tokcaer-Keskin Z, Tang Z, Strino F, Chen X, Wang Y, Xi X, Basilico C, Brown S, Bonneau R, Kluger Y, Dailey L. Nat Methods. 2014 May;11(5):559-65. doi: 10.1038/nmeth.2885. Epub 2014 Mar 23. 10.1038/nmeth.2885 PubMed 24658142