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6xO/S luc
(Plasmid #69445)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69445 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PGL-3 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5237
  • Modifications to backbone
    inserted 6XOS cassette and minimal fgf4 promoter upstream of luciferase coding sequences in PGL3 basic
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    448

Resource Information

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xO/S luc was a gift from Lisa Dailey (Addgene plasmid # 69445 ; http://n2t.net/addgene:69445 ; RRID:Addgene_69445)