MCJ.559
(Bacterial strain
#69493)
No maps are available for this item.
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PurposeStrain with recoded TAG and genomic deletions for improved incorporation
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 69493 | Bacteria in agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneStrain
- Backbone size w/o insert (bp) 1
- Total vector size (bp) 1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)MG1655
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Growth instructionsDo not grow above 34 oC. Lambda red cassette activated at 42 oC.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMG1655 based strain
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SpeciesEschericia coli
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Insert Size (bp)1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCJ.559 was a gift from Michael Jewett (Addgene plasmid # 69493) -
For your References section:
Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1. Hong SH, Kwon YC, Martin RW, Des Soye BJ, de Paz AM, Swonger KN, Ntai I, Kelleher NL, Jewett MC. Chembiochem. 2015 Mar 23;16(5):844-53. doi: 10.1002/cbic.201402708. Epub 2015 Mar 3. 10.1002/cbic.201402708 PubMed 25737329