pZDonor-U6-myogenin stop codon gRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69549||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMyogenin C terminus-targeting guide RNA
gRNA/shRNA sequenceMyogenin stop codon
SpeciesM. musculus (mouse)
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
- 3′ cloning site BbsI (destroyed during cloning)
- 5′ sequencing primer N/A
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Compatible with S. pyogenes Cas9.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZDonor-U6-myogenin stop codon gRNA was a gift from Charles Gersbach (Addgene plasmid # 69549 ; http://n2t.net/addgene:69549 ; RRID:Addgene_69549)