pBlueRIP.OVA
(Plasmid
#69597)
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Purposecontains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)
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Depositing Lab
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Depositing OrganizationMonash University
Located in Australia -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 69597-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 69597-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBlueRIP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOVA
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Alt namemembrane bound form of ovalbumin (OVA)
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SpeciesG. gallus (chicken)
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Insert Size (bp)1900
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Entrez GeneOVAL (a.k.a. OVA, SERPINB14)
- Promoter rat insulin promoter (RIP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBluescript-KS
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 69597-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 69597-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBlueRIP.OVA was a gift from Francis R. Carbone (Addgene plasmid # 69597 ; http://n2t.net/addgene:69597 ; RRID:Addgene_69597) -
For your References section:
Induction of autoimmune diabetes by oral administration of autoantigen. Blanas E, Carbone FR, Allison J, Miller JF, Heath WR. Science. 1996 Dec 6;274(5293):1707-9. PubMed 8939860
Map uploaded by the depositor.
