CYP2C8
(Plasmid #69604)

• Purpose: CYP2C8 cDNA IN PCw ORI+
• Depositing Lab: Joyce Goldstein
• Publication: Goldstein CYP2C9 and CYP2C9 in pOri+ for bacterial expression (unpublished)

Backbone

• Vector backbone: pCW
• Backbone size w/o insert (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYP2C8
• Species: H. sapiens (human)
• Entrez Gene: CYP2C8 (a.k.a. CPC8, CYPIIC8, MP-12/MP-20)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: pCW-SEQ-fwd (5'-ACATCGTATAACGTTACTGG-3')
• 3′ sequencing primer: pCW-SEQ-rev (5'-CTTTCGTCTTCAAGCAGATCTG-3')

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Modification of CYP2C8 for bacterial expression as per Barnes et al. PNAS 88:5597, 1991 [PMID: 1829523]: First 8 amino acids of CYP2C cDNAs replaced with those of bovine 17α hydroxylase (MALLLAVF) to optimize bacterial expression. An XbaI site was placed after the stop codon The insert was ligated into NdeI and XBbaI cut pCW ori+.