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pBS-L1PA8WTmneo
(Plasmid #69609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69609 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 69609-D50 50 μg of DNA in Tris buffer $495
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DNA 69609-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3530
  • Total vector size (bp) 11568
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; G418 detects retrotransposition not the plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Any standard E. coli strain should work fine.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L1PA8
  • Alt name
    LINE-1 PA8 subfamily (wild type sequence)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8027
  • Promoter CMV
  • Tag / Fusion Protein
    • mneo retrotransposition cassette

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ORF1 and ORF2 of the L1PA8 element were commercially synthetically generated and then cloned into the parental pBS-L1PA1CHmneo by swapping the two ORFs

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-L1PA8WTmneo was a gift from Astrid Roy-Engel (Addgene plasmid # 69609 ; http://n2t.net/addgene:69609 ; RRID:Addgene_69609)

  • For your References section:

    Molecular reconstruction of extinct LINE-1 elements and their interaction with nonautonomous elements. Wagstaff BJ, Kroutter EN, Derbes RS, Belancio VP, Roy-Engel AM. Mol Biol Evol. 2013 Jan;30(1):88-99. doi: 10.1093/molbev/mss202. Epub 2012 Aug 23. 10.1093/molbev/mss202 PubMed 22918960
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