pKG139
(Plasmid #69755)

• Purpose: Retroviral expression of CENP-C
• Depositing Lab: Iain Cheeseman
• Publication: Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10.1016/j.cell.2011.03.031.

Backbone

• Vector backbone: pIC194
• Backbone manufacturer: Arshad Desai (Addgene plasmid# 44433)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CENP-C
• Species: H. sapiens (human)
• Insert Size (bp): 2832
• Mutation: L341F, N570D relative to NP_001803.2 (numbering based on CENP-C without tag)
• Entrez Gene: CENPC (a.k.a. CENP-C, CENPC1, MIF2, hcp-4)
• Tag / Fusion Protein:
  • mCherry-TEV-S (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: mCherry-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Discrepancies between the plasmid and the NCBI reference sequence for CENP-C exist, however, the plasmid is predicted to function as described in the associated publication.