pEGFP-N1 VAMP2 R125TAG
(Plasmid #69876)

• Purpose: express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP
• Depositing Lab: Silvio Rizzoli
• Publication: Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. Epub 2015 Oct 28.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 5068
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP2-GFP
• Alt name: synaptobrevin 2
• Species: R. norvegicus (rat)
• Insert Size (bp): 1119
• Mutation: Amber stop codon in the linker between VAMP2 and GFP (R125TAG)
• Entrez Gene: Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry