pN1 VAMP2 R125TAG
(Plasmid
#69877)
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Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2
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Depositing Lab
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Depositing OrganizationUniversitaetsmedizin Goettingen
Located in Germany -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 69877-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 69877-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Total vector size (bp) 4328
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVAMP2
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Alt namesynaptobrevin 2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)378
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MutationAmber and Ochre stop codons in the former linker between VAMP2 and GFP (R125TAG and A126TAA)
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Entrez GeneVamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 69877-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 69877-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pN1 VAMP2 R125TAG was a gift from Silvio Rizzoli (Addgene plasmid # 69877 ; http://n2t.net/addgene:69877 ; RRID:Addgene_69877) -
For your References section:
Super-resolution Microscopy of Clickable Amino Acids Reveals the Effects of Fluorescent Protein Tagging on Protein Assemblies. Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell SW, Lemke EA, Rizzoli SO. ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. Epub 2015 Oct 28. 10.1021/acsnano.5b04434 PubMed 26498474
Map uploaded by the depositor.
