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pMY200
(Plasmid #69946)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69946 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR was used to amplify a fragment containing the left T-DNA border from PBht2. Two primers were designed as follows:
TRDNAS: CAAAgTTggCgTATAACATAgT, downstream of SacII site in pBht2.
TLDNAAPmeI: ATTATAgTTTAAACATTCggCgTTAATTCAgTACA, upstream of T-border 5’ end contains PmeI site in pBht2.

2. Digested PCR product with PmeI and SacII, gel extraction followed by recovery of ~400 bp fragment, and pBht2 vector was digested with PmeI and SacII, gel extracted, followed by recovery of ~6 kb fragment. CIP dephosphatase treatment, ligated with PCR product, electroporate into E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMY200 was a gift from Mark Farman (Addgene plasmid # 69946 ; http://n2t.net/addgene:69946 ; RRID:Addgene_69946)