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PurposeExpression of shRNA to human FOXA1, puromycin selection
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70095 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLKO_TRC005
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Backbone manufacturerBroad Institute
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 7000
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFOXA1
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gRNA/shRNA sequenceTCTAGTTTGTGGAGGGTTATT
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SpeciesH. sapiens (human)
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GenBank IDNM_004496.3
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Entrez GeneFOXA1 (a.k.a. HNF3A, TCF3A)
- Promoter U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pLKO_forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBroad Institute
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Broad Institute Clone ID: TRCN0000358367
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO_FOXA1_#1 was a gift from William Hahn (Addgene plasmid # 70095 ; http://n2t.net/addgene:70095 ; RRID:Addgene_70095) -
For your References section:
The androgen receptor cistrome is extensively reprogrammed in human prostate tumorigenesis. Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, Kantoff PW, Brown M, Loda M, Long HW, Freedman ML. Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 Oct 12. 10.1038/ng.3419 PubMed 26457646
