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Addgene

pEMS2157
(Plasmid #70118)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70118 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    AAV2
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ssAAV-MCS-EmGFP-WPRE
  • Alt name
    EmGFP
  • Species
    Synthetic
  • Promoter Promoterless

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site see map (unknown if destroyed)
  • 3′ cloning site see map (unknown if destroyed)
  • 5′ sequencing primer M13pUC-rev
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2157 was a gift from Elizabeth Simpson (Addgene plasmid # 70118 ; http://n2t.net/addgene:70118 ; RRID:Addgene_70118)
  • For your References section:

    New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914