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pRL1058
(Plasmid #70686)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70686 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Transposon Tn5, variously modified
  • Modifications to backbone
    5395 bp of the 6489 bp are a transposon whose ends are linked by an origin of transfer.
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Streptamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Transposon Tn5-1058

Resource Information

  • Terms and Licenses

Depositor Comments

Tn5 derivative Tn5-1058 (in plasmid pRL1058) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 is this series of plasmids), providing large numbers of transposon mutants. The XbaI site near the L end of Tn5 is the sole unmethylated XbaI in the construct, allowing the placement of luciferases (e.g., Vibrio fischeri luxAB as in pRL1063a). An oriT for conjugal transfer is positioned between the L and R ends of the Tn.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL1058 was a gift from Peter Wolk (Addgene plasmid # 70686 ; http://n2t.net/addgene:70686 ; RRID:Addgene_70686)
  • For your References section:

    Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium. Wolk CP, Cai Y, Panoff JM. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9. PubMed 11607193