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5n1 (560)
(Plasmid #70730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70730 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 70730-D50 50 μg of DNA in Tris buffer $495
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DNA 70730-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    minimize passages; can accumulate deletion mutations
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    srpR-ssrA

Gene/Insert 2

  • Gene/Insert name
    lambdaCI-ssrA

Gene/Insert 3

  • Gene/Insert name
    phlF-ssrA

Gene/Insert 4

  • Gene/Insert name
    lacI-ssrA

Gene/Insert 5

  • Gene/Insert name
    tetR-ssrA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5n1 (560) was a gift from Richard Murray (Addgene plasmid # 70730 ; http://n2t.net/addgene:70730 ; RRID:Addgene_70730)

  • For your References section:

    Rapid cell-free forward engineering of novel genetic ring oscillators. Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, Maerkl SJ. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771. 10.7554/eLife.09771 PubMed 26430766
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