PurposeNegative feedback plasmid from oscillator
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48882||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepZ vector
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namelacI with ssrA tag
Insert Size (bp)1122
- Promoter Para/lac
/ Fusion Protein
- ssrA (TSAANDENYALAA) (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer no info
- 3′ sequencing primer no info (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJS169 was a gift from Jeff Hasty (Addgene plasmid # 48882 ; http://n2t.net/addgene:48882 ; RRID:Addgene_48882)
For your References section:A fast, robust and tunable synthetic gene oscillator. Stricker J, Cookson S, Bennett MR, Mather WH, Tsimring LS, Hasty J. Nature. 2008 Nov 27;456(7221):516-9. doi: 10.1038/nature07389. Epub 2008 Oct 29. 10.1038/nature07389 PubMed 18971928