p2R3a-VenusYFP-3AT
(Plasmid #71269)

• Purpose: Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
• Depositing Lab: Ari Pekka Mähönen
• Publication: Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.

Backbone

• Vector backbone: p2RP3-pGEMteasy
• Backbone manufacturer: ampicillin resistant variant of pDONR-P2R-P3. attP2R-ccdB-cmR-attP3 sequences of pDONR-P2R-P3 were cloned into pGEMt-easy
• Vector type: Gateway

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VenusYFP
• Tags / Fusion Proteins:
  • 4xGly linker (N terminal on insert)
  • T3A pea Pisum sativum ribulose-1,5-bisphosphate carboxylase 3A subunit terminator. (Fluhr et al., 1986) (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-Fwd

Resource Information

• Supplemental Documents:
  • p2R3a-VenusYFP-3AT genbank
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry