HAI
(Plasmid
#71316)
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PurposeInducible expression of human arginase I in E. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBS(KS)
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Backbone manufacturerStratagene
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehuman arginase I
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Alt nameHAI
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Insert Size (bp)966
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Entrez GeneARG1
- Promoter tac
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFrancisco Centeno
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HAI was a gift from David Christianson (Addgene plasmid # 71316 ; http://n2t.net/addgene:71316 ; RRID:Addgene_71316) -
For your References section:
Crystal structure of human arginase I at 1.29-A resolution and exploration of inhibition in the immune response. DiCostanzo L, Sabio G, Mora A, Rodriguez PC, Ochoa AC, Centeno F, Christianson DW. Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13058-63. Epub 2005 Sep 2. 10.1073/pnas.0504027102 PubMed 16141327