p1D4-hrGFP II
(Plasmid
#71317)
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Purpose(Empty Backbone) expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepIRES-hrGFP II
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Backbone manufacturerStratagene
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Modifications to backbone1D4 epitope added
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- 1D4 epitope (TETSQVAPA) (C terminal on backbone)
- IRES-hrGFPII (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBased on "pIRES=hrGFP II" from Agilent Technologies. Catalog #240157
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p1D4-hrGFP II was a gift from Belinda Chang (Addgene plasmid # 71317 ; http://n2t.net/addgene:71317 ; RRID:Addgene_71317) -
For your References section:
The p1D4-hrGFP II expression vector: a tool for expressing and purifying visual pigments and other G protein-coupled receptors. Morrow JM, Chang BS. Plasmid. 2010 Nov;64(3):162-9. doi: 10.1016/j.plasmid.2010.07.002. Epub 2010 Jul 11. 10.1016/j.plasmid.2010.07.002 PubMed 20627111
Map uploaded by the depositor.
