PurposeExpresses TET1 with a mutated catalytic domain in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83569||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 11908
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)NEB Stable
Growth instructionsStbl3 bacteria should be used for transformation and plasmid should be grown at 37 for extended periods.
Gene/Insert nameTet Methylcytosine Dioxygenase 1
SpeciesH. sapiens (human)
Insert Size (bp)6408
Mutationcatalytic domain mutant H1672D, D1674A
Entrez GeneTET1 (a.k.a. CXXC6, LCX, bA119F7.1)
- Promoter CMV
/ Fusion Proteins
- 3X FLAG tag (C terminal on backbone)
- hr GFP II (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer ATGGGCGGTAGGCGTGTA
- 3′ sequencing primer ATGCAGTCGTCGAGGAATTG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Mutation location described in the following publication: Minimal role of base excision repair in TET-induced global DNA demethylation in HEK293T cells, EPIGENETICS 2015
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIRES-hrGFP II-mTET1 was a gift from Jean-Pierre Issa (Addgene plasmid # 83569 ; http://n2t.net/addgene:83569 ; RRID:Addgene_83569)
For your References section:TET1 is a maintenance DNA demethylase that prevents methylation spreading in differentiated cells. Jin C, Lu Y, Jelinek J, Liang S, Estecio MR, Barton MC, Issa JP. Nucleic Acids Res. 2014 Jun;42(11):6956-71. doi: 10.1093/nar/gku372. Epub 2014 May 29. 10.1093/nar/gku372 PubMed 24875481