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pSTARR-seq_fly-NipB
(Plasmid #71502)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71502 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Promoter
  • Backbone manufacturer
    Promega
  • Vector type
    Insect Expression ; flySTARR-seq screening vector
  • Tag / Fusion Protein
    • sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • sgGFP (SuperGlo, Qbiogene, Inc) (N terminal on backbone)

Resource Information

Depositor Comments

This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSTARR-seq_fly-NipB was a gift from Alexander Stark (Addgene plasmid # 71502)
  • For your References section:

    Enhancer-core-promoter specificity separates developmental and housekeeping gene regulation. Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, Stark A. Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 2014 Dec 15. 10.1038/nature13994 PubMed 25517091