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pGL3-Gateway-DSCP
(Plasmid #71506)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71506 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Promoter
  • Backbone manufacturer
    Promega
  • Vector type
    Insect Expression ; flySTARR-seq validation vector
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Resource Information

Depositor Comments

We replaced the SV40 promotor of the pGL3-promotor plasmid by DSCP and inserted a Gateway-cassette into the multiple-cloning-site (MCS; using KpnI and BglII), i.e. upstream of the DSCP, to allow Gateway cloning of candidate sequences

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Gateway-DSCP was a gift from Alexander Stark (Addgene plasmid # 71506)
  • For your References section:

    Genome-wide quantitative enhancer activity maps identified by STARR-seq. Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A. Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. Epub 2013 Jan 17. 10.1126/science.1232542 PubMed 23328393