Purpose(Empty Backbone) Cloning vector for transient expression of sgRNA-5xPBSb
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71891||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepX330; pUC ori vector
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Unknown
- 5′ sequencing primer gagggcctatttcccatgattcc
- 3′ sequencing primer gccatttgtctgcagaattggc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC1374-pX-sgRNA-5xPBSb was a gift from Albert Cheng (Addgene plasmid # 71891 ; http://n2t.net/addgene:71891 ; RRID:Addgene_71891)
For your References section:Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. 10.1038/cr.2016.3 PubMed 26768771