PurposeExpresses the extracellular region of the Sema5B protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72036||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameSemaphorin 5B; SemG; Semag
Alt nameNCBI gene ID 20357; Reference sequence NM_013661.2
SpeciesM. musculus (mouse)
Insert Size (bp)2928
Entrez GeneSema5b (a.k.a. AI893641, SemG, Semag, mKIAA1445)
- Promoter CMV
/ Fusion Protein
- AP-His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer GTCAGAGGTAACTCCCGTTGC
- 3′ sequencing primer GAGGTTCTTGGCGGCTGTC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene Sanger sequencing found Y62H compared to GenBank ID NM_013661.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Sema5b-AP-His was a gift from Woj Wojtowicz (Addgene plasmid # 72036 ; http://n2t.net/addgene:72036 ; RRID:Addgene_72036)
For your References section:An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM. Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149. 10.7554/eLife.08149 PubMed 26633812