PurposeExpresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72166||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameSemaphorin 6B; VIb; Sema; Seman; semaZ
Alt nameNCBI gene ID 20359; Reference sequence NM_013662.2
SpeciesM. musculus (mouse)
Insert Size (bp)1455
Entrez GeneSema6b (a.k.a. Sema, Seman, VIb, semaZ)
- Promoter CMV
/ Fusion Protein
- Fc-His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer GTCAGAGGTAACTCCCGTTGC
- 3′ sequencing primer ATCATGAGGGTGTCCTTG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene Sanger sequencing found F187L within the Sema6b translation compared to NM_013662.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Sema6b(S)-Fc-His was a gift from Woj Wojtowicz (Addgene plasmid # 72166 ; http://n2t.net/addgene:72166 ; RRID:Addgene_72166)
For your References section:An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM. Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149. 10.7554/eLife.08149 PubMed 26633812