|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72365||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5771
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameDNMT3B Homology Arms
SpeciesH. sapiens (human)
Entrez GeneDNMT3B (a.k.a. FSHD4, ICF, ICF1, M.HsaIIIB)
/ Fusion Protein
- 3XFLAG-P2A-NeoR (C terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer Neo-F
- 3′ sequencing primer Neo-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFETCh_DNMT3B was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 72365 ; http://n2t.net/addgene:72365 ; RRID:Addgene_72365)