Purpose(Empty Backbone) Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72647||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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- Backbone size (bp) 8250
Modifications to backboneLE clonase Gateway compatible
Vector typesucrose counter-selection allelic exchange vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Tetracycline, 25 & 10 μg/mL
Growth instructionsThe strain grows to a lower final turbidity than other E coli strains and is cold sensitive. Do not store @ 4C.
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer TGTAAAACGACGGCCAGT
- 3′ sequencing primer AACAGCTATGACATGA (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDEST2T18ms was a gift from Brian Kvitko (Addgene plasmid # 72647 ; http://n2t.net/addgene:72647 ; RRID:Addgene_72647)