pLicC-MBP-APETx2
(Plasmid #72668)

• Purpose: Periplasmic expression of APETx2 in E.coli
• Depositing Lab: Glenn King
• Publication: Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi: 10.3390/md10071605. Epub 2012 Jul 23.

Backbone

• Vector backbone: pLicC-MBP
• Backbone manufacturer: Geneart (Invitrogen)
• Total vector size (bp): 6554
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: APETx2
• Species: Sea anemone
• Insert Size (bp): 200
• Promoter: T7
• Tags / Fusion Proteins:
  • MalE signal sequence for periplasmic export (N terminal on backbone)
  • His tag (N terminal on backbone)
  • MBP (N terminal on backbone)
  • TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV/KpnI (not destroyed)
• 3′ cloning site: SacI/SalI/HindIII/NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Purchased from Genart
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry