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pGL4.10 Dub3
(Plasmid #72684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72684 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 72684-D50 50 μg of DNA in Tris buffer $495
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DNA 72684-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL4.10[luc]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dub3 promoter
  • Alt name
    ubiquitin specific peptidase 17-like E
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3200
  • Entrez Gene
    Usp17le (a.k.a. DUB-3, Dub3, Dub6, EG625530, Gm6596, USP17, USP17-E, Usp17l2)
  • Promoter Dub3
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found a small number of single nucleotide discrepancies relative to Sanger sequencing results provided by the depositing laboratory. These do not affect plasmid function

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.10 Dub3 was a gift from Domenico Maiorano (Addgene plasmid # 72684 ; http://n2t.net/addgene:72684 ; RRID:Addgene_72684)

  • For your References section:

    Cell cycle-dependent expression of Dub3, Nanog and the p160 family of nuclear receptor coactivators (NCoAs) in mouse embryonic stem cells. van der Laan S, Golfetto E, Vanacker JM, Maiorano D. PLoS One. 2014 Apr 2;9(4):e93663. doi: 10.1371/journal.pone.0093663. eCollection 2014. PONE-D-14-02572 [pii] PubMed 24695638
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