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pCfB3040(XII-4 MarkerFree)
(Plasmid #73280)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73280 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p0130 (pXII-4-DR-KlURA3)
  • Backbone manufacturer
    DTU
  • Backbone size (bp) 4435
  • Modifications to backbone
    The pCfB3040 vector was created by amplifying the p0130 (pXII-4-DR-KlURA3) with primers that were designed to attach to either side of the selection markers, creating a fragment that no longer contained the marker. These fragments were then ligated to form the marker-less vectors. The PAM sites in the integration regions were removed by site-directed mutagenesis.
  • Vector type
    Yeast Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfB3040(XII-4 MarkerFree) was a gift from Irina Borodina (Addgene plasmid # 73280 ; http://n2t.net/addgene:73280 ; RRID:Addgene_73280)
  • For your References section:

    EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, Keasling JD, Borodina I. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23. 10.1002/biot.201600147 PubMed 27166612